1973 Articles

Isolation and characterization of revertant cell lines. IV. Direct selection of serum‐revertant sublines of SV40‐transformed 3T3 mouse cells

Vogel, A.; Pollack, Robert

SV101, the SV40‐transformed subline of the mouse fibroblast line 3T3, is both serum‐ and density transformed, since it grows in both 1% and 10% calf serum, and grows beyond confluence in 10% calf serum. Negative selection at low cell density in 1% calf serum or in 10% agamma‐depleted serum permits direct recovery of serum‐revertant sublines of SV101. These sublines are unable to grow in 1% calf serum. Although negative selection at high cell density in 10% calf serum is known to permit recovery of density‐revertant sublines of SV101, most density‐revertants are not serum‐revertant. However, all serum‐revertants isolated so far are density‐revertant as well.