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Genomic and Expression Analysis of the 12p11-p12 Amplicon Using EST Arrays Identifies Two Novel Amplified and Overexpressed Genes

Bourdon, Veronique; Naef, Félix; Rao, Pulivarthi; Reuter, Victor; Mok, Samuel C.; Bosl, George; Koul, Sanjay; Vundavalli, Murty V.; Kucherlapati, Raju; Chaganti, R.S.K.

We performed parallel array comparative genomic hybridization and array expression analysis of the 12p11-p12 amplicon in human testicular seminomas and an ovarian carcinoma cell line using an expressed se- quence tags (ESTs) array spotted with 8254 ESTs. The data were normal- ized using a robust statistical modeling and the significance inferred from the local SD. We identified two ESTs within the chromosomal amplicon that were amplified and overexpressed in >75–100% of analyzed tumors with the 12p11-p12 amplicon. These sequences, belonging to coding re- gions of two novel genes designated here as GCT1 and GCT2, were broadly expressed in a panel of human tissues, including testis and ovary. GCT1 and GCT2 were overexpressed in 92 and 71%, respectively, of a panel of seminomas tested. Combined array comparative genomic hybridization and array expression analysis is a valid approach for gene discovery in large chromosomal amplicons.

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Cancer Research

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Academic Units
Pathology and Cell Biology
Published Here
February 24, 2020