Academic Commons

Theses Doctoral

New Tools for Understanding and Engineering Complex Microbial Communities

Sheth, Ravi Uday

Microbes exist in unfathomably diverse, dynamic and intricately structured ecosystems. However, we lack the tools to fully capture the complexity of these microbiomes, which in turn limits our ability to understand their ecology and function. Here, I address these shortcomings by developing new high-throughput measurement tools to characterize microbiomes across functionally distinct axes. First, from a synthetic biology perspective, I leverage the bacterial CRISPR-Cas immune system to enable a new class of population-wide passive recording devices in cells for capture temporally varying signals and horizontally transferred DNA sequences. Second, in the microbiome arena, I develop a new suite of tools (experimental and theoretical) to capture and analyze the spatiotemporal dynamics of microbiomes at macroscopic and microscopic length scales. Taken together, these measurements provide deep insights into the ecology of complex microbiomes, and constitute a suite of powerful new tools to study microbes in their native context.

Files

  • thumnail for Sheth_columbia_0054D_15589.pdf Sheth_columbia_0054D_15589.pdf application/pdf 34.8 MB Download File

More About This Work

Academic Units
Cellular, Molecular and Biomedical Studies
Thesis Advisors
Wang, Harris H.
Degree
Ph.D., Columbia University
Published Here
October 28, 2019
Academic Commons provides global access to research and scholarship produced at Columbia University, Barnard College, Teachers College, Union Theological Seminary and Jewish Theological Seminary. Academic Commons is managed by the Columbia University Libraries.