1974 Articles

Biological analysis of clones of SV40-infected mouse 3T3 cells

Risser, Rex; Robert,, Pollack E.

A variety of selective assays have been used to monitor the transformation of normal cells into tumor cells. These assays take advantage of the ability of transformed cells to grow to high saturation densities (Todaro, Green and Goldberg 1964), to grow in the absence of anchorage to glass or plastic (Macpherson and Montagnier 1964; Stoker et al. 1968), to form colonies or foci on monolayers of normal cells (Temin and Rubin 1958), to grow in reduced concentrations of serum components (Holley and Kiernan 1968; Jainchill and Todaro 1970; Smith, Scher and Todaro 1971), and to grow in disoriented patterns (Stoker and Abel 1962).