Detection of tick-borne pathogens by MassTag polymerase chain reaction

Tokarz, Rafal; Kapoor, Vishal; Samuel, James E.; Bouyer, Donald H.; Briese, Thomas; Lipkin, W. Ian

MassTag polymerase chain reaction (PCR) is a platform that enables microbe detection using primers labeled through a photocleavable link with tags that vary in molecular weight. After multiplex PCR, tags are released by ultraviolet irradiation and analyzed by mass spectroscopy. The identification of a microbe in a sample is determined by its cognate tags. Here we describe establishment and implementation of a MassTag PCR panel for surveillance of microbes implicated in tick-vectored infectious diseases.

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Vector-Borne and Zoonotic Diseases

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Academic Units
Center for Infection and Immunity
Published Here
February 26, 2013