Academic Commons

Articles

Expanding the Repertoire of Optogenetically Targeted Cells with an Enhanced Gene Expression System

Tanaka, Kenji F.; Matsui, Ko; Sasaki, Takuya; Sano, Hiromi; Sugio, Shouta; Fan, Kai; Hen, Rene; Nakai, Junichi; Yanagawa, Yuchio; Hasuwa, Hidetoshi; Okabe, Masaru; Deisseroth, Karl; Ikenaka, Kazuhiro; Yamanaka, Akihiro

Optogenetics has been enthusiastically pursued in recent neuroscience research, and the causal relationship between neural activity and behavior is becoming ever more accessible. Here, we established knockin-mediated enhanced gene expression by improved tetracycline-controlled gene induction (KENGE-tet) and succeeded in generating transgenic mice expressing a highly light-sensitive channelrhodopsin-2 mutant at levels sufficient to drive the activities of multiple cell types. This method requires two lines of mice: one that controls the pattern of expression and another that determines the protein to be produced. The generation of new lines of either type readily expands the repertoire to choose from. In addition to neurons, we were able to manipulate the activity of nonexcitable glial cells in vivo. This shows that our system is applicable not only to neuroscience but also to any biomedical study that requires understanding of how the activity of a selected population of cells propagates through the intricate organic systems.

Files

  • thumnail for 1-s2.0-S2211124712001714-main.pdf 1-s2.0-S2211124712001714-main.pdf application/pdf 1.75 MB Download File

Also Published In

More About This Work

Academic Units
Psychiatry
Publisher
Elsevier
Published Here
March 8, 2016
Academic Commons provides global access to research and scholarship produced at Columbia University, Barnard College, Teachers College, Union Theological Seminary and Jewish Theological Seminary. Academic Commons is managed by the Columbia University Libraries.