2011 Articles
Accurate proteome-wide protein quantification from high-resolution 15N mass spectra
In quantitative mass spectrometry-based proteomics, the metabolic incorporation of a single source of ¹⁵N-labeled nitrogen has many advantages over using stable isotope-labeled amino acids. However, the lack of a robust computational framework for analyzing the resulting spectra has impeded wide use of this approach. We have addressed this challenge by introducing a new computational methodology for analyzing ¹⁵N spectra in which quantification is integrated with identification. Application of this method to an Escherichia coli growth transition reveals significant improvement in quantification accuracy over previous methods.
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Also Published In
- Title
- Genome Biology
- DOI
- https://doi.org/10.1186/gb-2011-12-12-r122
More About This Work
- Academic Units
- Biochemistry and Molecular Biophysics
- Publisher
- BioMed Central
- Published Here
- September 8, 2014