Theses Doctoral

Identifying genes required for the formation of neurons from skin cells using forward genetic screens and whole genome sequencing in C. elegans

Minevich, Gregory

The human brain is the most complex structure in the known universe and one of the ultimate goals of humanity is to understand its function. The "bottom-up" approach to developmental neuroscience seeks to assemble a "parts list" of the genes expressed in each neuron and a map of the gene regulatory networks that determine the identity of the diverse neuronal types. A key part of building such a gene regulatory map is to identify the transcription factors that are key nodes in these networks.
The goal of my PhD was to study the particular gene regulatory networks that govern the decision of the V5 skin cell to divide, lose its skin fate and decide to make dopamine and glutamate sensory neurons. We chose an unbiased forward genetic screen approach coupled with whole genome sequencing of mutants derived from these screens. In the process, we found several mutants that govern this process and developed a software pipeline that simplifies the analysis of mutants for others who perform forward genetic screens.


  • thumnail for Minevich_columbia_0054D_12576.pdf Minevich_columbia_0054D_12576.pdf application/pdf 74.9 MB Download File

More About This Work

Academic Units
Pathobiology and Molecular Medicine
Thesis Advisors
Hobert, Oliver
Ph.D., Columbia University
Published Here
April 10, 2015