Academic Commons

Theses Doctoral

Chemical Approaches for Advanced Optical Imaging

Chen, Zhixing

Advances in optical microscopy have been constantly expanding our knowledge of biological systems. The achievements therein are a result of close collaborations between physicists/engineers who build the imaging instruments and chemists/biochemists who design the corresponding probe molecules. In this work I present a number of chemical approaches for the development of advanced optical imaging methods. Chapter 1 provides an overview of the recent advances of novel imaging approaches taking advantage of chemical tag technologies. Chapter 2 describes the second-generation covalent trimethoprim-tag as a viable tool for live cell protein-specific labeling and imaging. In Chapter 3 we present a fluorescence lifetime imaging approach to map protein-specific micro-environment in live cells using TMP-Cy3 as a chemical probe. In Chapter 4, we present a method harnessing photo-activatable fluorophores to extend the fundamental depth limit in multi-photon microscopy. Chapter 5 describes the development of isotopically edited alkyne palette for multi-color live cell vibrational imaging of cellular small molecules. These studies exemplify the impact of modern chemical approaches in the development of advanced optical microscopies.



  • thumnail for Chen_columbia_0054D_12232.pdf Chen_columbia_0054D_12232.pdf binary/octet-stream 6.27 MB Download File

More About This Work

Academic Units
Thesis Advisors
Cornish, Virginia
Ph.D., Columbia University
Published Here
July 7, 2014
Academic Commons provides global access to research and scholarship produced at Columbia University, Barnard College, Teachers College, Union Theological Seminary and Jewish Theological Seminary. Academic Commons is managed by the Columbia University Libraries.