2016 Articles
Development of a Rapid Insulin Assay by Homogenous Time-Resolved Fluorescence
Direct measurement of insulin is critical for basic and clinical studies of insulin secretion. However, current methods are expensive and time-consuming. We developed an insulin assay based on homogenous time-resolved fluorescence that is significantly more rapid and cost-effective than current commonly used approaches. This assay was applied effectively to an insulin secreting cell line, INS-1E cells, as well as pancreatic islets, allowing us to validate the assay by elucidating mechanisms by which dopamine regulates insulin release. We found that dopamine functioned as a significant negative modulator of glucose-stimulated insulin secretion. Further, we showed that bromocriptine, a known dopamine D2/D3 receptor agonist and newly approved drug used for treatment of type II diabetes mellitus, also decreased glucose-stimulated insulin secretion in islets to levels comparable to those caused by dopamine treatment.
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journal.pone.0148684.PDF application/pdf 1.66 MB Download File
Also Published In
- Title
- PLoS ONE
- DOI
- https://doi.org/10.1371/journal. pone.0148684
More About This Work
- Academic Units
- Center for Radiological Research
- Psychiatry
- Medicine
- Pharmacology
- Publisher
- Public Library of Science
- Published Here
- March 14, 2016