2012 Articles

Light dependence of phosphorus uptake by microorganisms in the subtropical North and South Pacific Ocean

Björkman, Karin M.; Duhamel, Solange; Karl, David M.

Light and dark phosphate (PO₄³⁻) uptake rates were investigated in the North Pacific Subtropical Gyre (NPSG) and along a coastal to open ocean transect in the South Pacific Ocean. PO₄³⁻ uptake rates were consistently higher when incubated in the light, but the ratio of uptake in the light and dark (L:D) decreased with depth. In the NPSG, the L:D ratio of euphotic-layer integrated PO₄³⁻ uptake was 1.58 ± 0.08 (±SE, n = 15 profiles), and the average L:D ratio was 1.60 ± 0.45 (±SE, n = 42) between 5 and 45 m and 1.25 ± 0.70 (±SE, n = 28) between 150 and 175 m. The L:D ratio was higher for the pigmented plankton-enriched size fractions (0.6 to 2 µm and >2 µm), but results were difficult to interpret at the oligotrophic stations where non-pigmented and pigmented plankton cell sizes overlapped. Group-specific measurements obtained using flow cytometric cell sorting demonstrated that Prochlorococcus PO₄³⁻ uptake rates were higher when the samples were incubated under ambient light. Adenosine-5′-triphosphate (ATP) uptake by Prochlorococcus was also higher in the light for the uptake of both the terminal PO₄³⁻ group ([γ‑³³P]ATP) and adenine moiety ([2,8-³H]ATP). This could be the result of secondary uptake of PO₄³⁻ and/or adenine after ATP cleavage by non-pigmented picoplankton. There was no significant difference in P-assimilation by non-pigmented picoplankton between light and dark incubated samples. Light dependence of phytoplankton PO₄³⁻ uptake could thus influence the functioning of the microbial loop and the flows of matter and energy in marine environments by creating temporal patterns of resource utilization.