Academic Commons

Articles

Molecular Cloning of a Bovine Immunoglobulin Lambda Chain cDNA

Ivanov, Vladimir N.; Gorodetsky, Stanislav I.; Karginov, Vladimir A.; Morozov, Igor V.

A cDNA library of the bovine mammary gland constructed in pBR322 was screened by mRNA hybrid-selected translation and by differential hybridization. Several immunoglobulin (Ig) λ light-chain clones were identified and sequenced. Nucleotide sequence comparison of bovine and human Ig λ chains showed a high degree of homology for constant regions and for J regions. The amino acid (aa) sequence encoded by the constant region of the bovine Ig λ chain cDNA contains 107 aa with differences at 24 aa positions from the human Ig A chain. Three complementarity-determining regions (CDR1,2,3) characteristic of the variable region of bovine Ig λ chain cDNA can be distinguished. The bovine and human sequences display good homology in the framework region 3 (FR3) but only patches of homology throughout the FR2 region. The 5′ end of the bovine Ig λ chain cDNA fragment of clone 1-14E contains five stop codons: two in CDR1, one in FR1 and two in the hydrophobic prepeptide region. These data suggest that the Igλ mRNA of clone 1-14E is transcribed from the Vλ pseudogene.

Files

  • thumnail for 1-s2.0-0378111988900066-main.pdf 1-s2.0-0378111988900066-main.pdf application/pdf 688 KB Download File

Also Published In

More About This Work

Academic Units
Center for Radiological Research
Publisher
Elsevier Science
Published Here
September 11, 2015
Academic Commons provides global access to research and scholarship produced at Columbia University, Barnard College, Teachers College, Union Theological Seminary and Jewish Theological Seminary. Academic Commons is managed by the Columbia University Libraries.