Academic Commons

Articles

Detailed Analysis of the African Green Monkey Model of Nipah Virus Disease

Johnston, Sara C.; Briese, Thomas; Pratt, William D.; Bell, Todd M.; Shamblin, Joshua D.; Esham, Heather L.; Donnelly, Ginger C.; Johnson, Joshua C.; Hensley, Lisa E.; Lipkin, Ian W.; Honko, Anna N.

Henipaviruses are implicated in severe and frequently fatal pneumonia and encephalitis in humans. There are no approved vaccines or treatments available for human use, and testing of candidates requires the use of well-characterized animal models that mimic human disease. We performed a comprehensive and statistically-powered evaluation of the African green monkey model to define parameters critical to disease progression and the extent to which they correlate with human disease. African green monkeys were inoculated by the intratracheal route with 2.5×104 plaque forming units of the Malaysia strain of Nipah virus. Physiological data captured using telemetry implants and assessed in conjunction with clinical pathology were consistent with shock, and histopathology confirmed widespread tissue involvement associated with systemic vasculitis in animals that succumbed to acute disease. In addition, relapse encephalitis was identified in 100% of animals that survived beyond the acute disease phase. Our data suggest that disease progression in the African green monkey is comparable to the variable outcome of Nipah virus infection in humans.

Files

Also Published In

More About This Work

Academic Units
Center for Infection and Immunity
Published Here
February 2, 2016
Academic Commons provides global access to research and scholarship produced at Columbia University, Barnard College, Teachers College, Union Theological Seminary and Jewish Theological Seminary. Academic Commons is managed by the Columbia University Libraries.