Academic Commons

Articles

Microrobotic Streak Seeding for Protein Crystal Growth

Georgiev, Atanas; Allen, Peter K.; Song, Ting; Laine, Andrew F.; Edstrom, William; Hunt, John F.

We present a microrobotic system for protein crystal micromanipulation tasks. The focus in this paper is on the task known to crystallographers as streak seeding — it is used to entice certain protein crystals to grow. Our system features a set of custom designed micropositioner end-effectors we call microshovels to replace traditional tools used by crystallographers for this task. We have used micro-electrical mechanical system (MEMS) techniques to design and manufacture various shapes and quantities of microshovels. Visual input from a camera mounted on the microscope is used to detect the locations of the source crystals which the tool needs to touch as well as the locations of the target protein droplets for seeding. We present experimental results that illustrate the applicability of our approach.

Files

More About This Work

Academic Units
Biomedical Engineering
Published Here
August 13, 2010

Notes

Robotics: Science and Systems I (Cambridge, Mass.: MIT Press, 2005), pp. 137-144.

Academic Commons provides global access to research and scholarship produced at Columbia University, Barnard College, Teachers College, Union Theological Seminary and Jewish Theological Seminary. Academic Commons is managed by the Columbia University Libraries.