Academic Commons

Theses Doctoral

The Ribosome Discriminates the Structure of the Amino Acid at its Peptidyl-Transferase Center

Englander, Michael Thomas

Contemporary interpretations of Crick's original Adaptor Hypothesis view both the amino acid and the tRNA body as passive participants during aminoacyl-tRNA (aa-tRNA) selection by the ribosome. Recent experimental evidence investigating tRNA mutants that miscode due to mutations that lie outside the anticodon as well as data from the unnatural amino acid mutagenesis field that shows that different unnatural amino acids, when esterified to the same suppressor tRNA, produce different amounts of protein, suggest that aa-tRNA selection is considerably more complex than originally envisioned in Crick's Adaptor Hypothesis and that aa-tRNA selection may extend to the amino acid itself. Here, using unnatural amino acids as substrates on natural, fully modified tRNAs and a highly purified in vitro translation system, we investigate the substrate specificity of the ribosome with respect to the amino acid.

Subjects

Files

  • thumnail for Englander_columbia_0054D_10339.pdf Englander_columbia_0054D_10339.pdf application/pdf 5.71 MB Download File

More About This Work

Academic Units
Cellular, Molecular and Biomedical Studies
Thesis Advisors
Cornish, Virginia W.
Degree
Ph.D., Columbia University
Published Here
June 28, 2013
Academic Commons provides global access to research and scholarship produced at Columbia University, Barnard College, Teachers College, Union Theological Seminary and Jewish Theological Seminary. Academic Commons is managed by the Columbia University Libraries.