Academic Commons

Articles

ER-mitochondria tethering by PDZD8 regulates Ca2+ dynamics in mammalian neurons

Hirabayashi, Yusuke; Kwon, Seok-Kyu; Paek, Hunki; Pernice, Wolfgang Maximilian; Paul, Maëla A.; Lee, Jinoh; Erfani, Parsa; Raczkowski, Ashleigh; Petrey, Donald S.; Pon, Liza A.; Polleux, Franck

Interfaces between organelles are emerging as critical platforms for many biological responses in eukaryotic cells. In yeast, the ERMES complex is an endoplasmic reticulum (ER)-mitochondria tether composed of four proteins, three of which contain a SMP (synaptotagmin-like mitochondrial-lipid binding protein) domain. No functional ortholog for any ERMES protein has been identified in metazoans. Here, we identified PDZD8 as an ER protein present at ER-mitochondria contacts. The SMP domain of PDZD8 is functionally orthologous to the SMP domain found in yeast Mmm1. PDZD8 was necessary for the formation of ER-mitochondria contacts in mammalian cells. In neurons, PDZD8 was required for calcium ion (Ca2+) uptake by mitochondria after synaptically induced Ca2+-release from ER and thereby regulated cytoplasmic Ca2+ dynamics. Thus, PDZD8 represents a critical ER-mitochondria tethering protein in metazoans. We suggest that ER-mitochondria coupling is involved in the regulation of dendritic Ca2+ dynamics in mammalian neurons.

Files

  • thumnail for Hirabayashi_Science2017_plusSM.pdf Hirabayashi_Science2017_plusSM.pdf application/pdf 7.73 MB Download File

Also Published In

More About This Work

Academic Units
Neuroscience
Published Here
November 8, 2018