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Chemosensitivity assay in mice prostate tumor: Preliminary report of flow cytometry, DNA fragmentation, ion ratiometric methods of anti-neoplastic drug monitoring

Sharma, Rakesh; Kline, Richard P.

PC-3 cell lines were compared with DU-145, LNCaP cell lines in culture for the [Na]i and [Ca]i ion sensing dyes, cell death, NMR peaks and apoptosis staining for chemotherapeutic action of different drugs. DNA fragmentation, ratiometric ions and fluorescence endlabelling plots were characteristic for cell lines and drug response. 31P-23Na NMR spectra showed characteristic high phospho-choline and sodium peaks. Flow cytometry, DNA fragmentation, ion ratiometric methods and NMR peaks indicated apoptosis and offered in vivo drug monitoring method.

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Also Published In

Title
Cancer Cell International
DOI
https://doi.org/10.1186/1475-2867-4-3

More About This Work

Academic Units
Radiology
Publisher
BioMed Central
Published Here
September 8, 2014

Notes

MRI, apoptosis, prostate tumor, flow cytometry, ion ratiometric assay, DNA fragmentation