A bidirectional relationship between sleep and oxidative stress in Drosophila

Hill, Vanessa Maria; O’Connor, Reed M.; Sissoko, Gunter B.; Irobunda, Ifeoma S.; Leong, Stephen; Canman, Julie; Stavropoulos, Nicholas; Shirasu-Hiza, Mimi M.

Although sleep appears to be broadly conserved in animals, the physiological functions of sleep remain unclear. In this study, we sought to identify a physiological defect common to a diverse group of short-sleeping Drosophila mutants, which might provide insight into the function and regulation of sleep. We found that these short-sleeping mutants share a common phenotype of sensitivity to acute oxidative stress, exhibiting shorter survival times than controls. We further showed that increasing sleep in wild-type flies using genetic or pharmacological approaches increases survival after oxidative challenge. Moreover, reducing oxidative stress in the neurons of wild-type flies by overexpression of antioxidant genes reduces the amount of sleep. Together, these results support the hypothesis that a key function of sleep is to defend against oxidative stress and also point to a reciprocal role for reactive oxygen species (ROS) in neurons in the regulation of sleep.


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Academic Units
Genetics and Development
Pathology and Cell Biology
Published Here
September 25, 2018