Academic Commons


Integrated circuit-based electrochemical sensor for spatially resolved detection of redox-active metabolites in biofilms

Bellin, Daniel L.; Sakhtah, Hassan; Thimot, Jordan; Emmett, Kevin; Dietrich, Lars; Shepard, Kenneth L.

Despite advances in monitoring spatiotemporal expression patterns of genes and proteins with fluorescent probes, direct detection of metabolites and small molecules remains challenging. A technique for spatially resolved detection of small molecules would benefit the study of redox-active metabolites that are produced by microbial biofilms and can affect their development. Here we present an integrated circuit-based electrochemical sensing platform featuring an array of working electrodes and parallel potentiostat channels. ‘Images’ over a 3.25 0.9 mm2 area can be captured with a diffusion-limited spatial resolution of 750 mm.
We demonstrate that square wave voltammetry can be used to detect, identify and quantify (for concentrations as low as 2.6 mM) four distinct redox-active metabolites called phenazines. We characterize phenazine production in both wild-type and mutant Pseudomonas aeruginosa PA14 colony biofilms, and find correlations with fluorescent reporter imaging of phenazine biosynthetic gene expression.


  • thumnail for Bellin_et_al-2014-Nature_Communications.pdf Bellin_et_al-2014-Nature_Communications.pdf application/pdf 1 MB Download File

Also Published In

Nature Communications

More About This Work

Academic Units
Electrical Engineering
Biological Sciences
Published Here
March 31, 2015
Academic Commons provides global access to research and scholarship produced at Columbia University, Barnard College, Teachers College, Union Theological Seminary and Jewish Theological Seminary. Academic Commons is managed by the Columbia University Libraries.