Integrated circuit-based electrochemical sensor for spatially resolved detection of redox-active metabolites in biofilms

Bellin, Daniel L.; Sakhtah, Hassan; Thimot, Jordan; Emmett, Kevin; Dietrich, Lars; Shepard, Kenneth L.

Despite advances in monitoring spatiotemporal expression patterns of genes and proteins with fluorescent probes, direct detection of metabolites and small molecules remains challenging. A technique for spatially resolved detection of small molecules would benefit the study of redox-active metabolites that are produced by microbial biofilms and can affect their development. Here we present an integrated circuit-based electrochemical sensing platform featuring an array of working electrodes and parallel potentiostat channels. ‘Images’ over a 3.25 0.9 mm2 area can be captured with a diffusion-limited spatial resolution of 750 mm.
We demonstrate that square wave voltammetry can be used to detect, identify and quantify (for concentrations as low as 2.6 mM) four distinct redox-active metabolites called phenazines. We characterize phenazine production in both wild-type and mutant Pseudomonas aeruginosa PA14 colony biofilms, and find correlations with fluorescent reporter imaging of phenazine biosynthetic gene expression.


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Nature Communications

More About This Work

Academic Units
Electrical Engineering
Biological Sciences
Published Here
March 31, 2015