Academic Commons

Articles

Molecular differences between stromal cell populations from deciduous and permanent human teeth

Kaukua, Nina; Chen, Mo; Guarnieri, Paolo; Dahl, Markus; Lim, Mei Ling; Yucel-Lindberg, Tülay; Sundström, Erik; Adameyko, Igor; Mao, Jeremy J.; Fried, Kaj

Introduction: Deciduous and permanent human teeth represent an excellent model system to study aging of stromal populations. Aging is tightly connected to self-renewal and proliferation and thus, mapping potential molecular differences in these characteristics between populations constitutes an important task. Methods: Using specifically designed microarray panels, Real-Time Quantitative Polymerase Chain Reaction (RT q-PCR), Western blot, immunohistochemistry and siRNA-mediated knock down experiments, we have detected a number of molecules that were differentially expressed in dental pulp from deciduous and permanent teeth extracted from young children and adults, respectively. Results: Among the differentially regulated genes, high-mobility group AT-hook 2 (HMGA2), a stem cell-associated marker, stood out as a remarkable example with a robust expression in deciduous pulp cells. siRNA-mediated knock down of HMGA2 expression in cultured deciduous pulp cells caused a down-regulated expression of the pluripotency marker NANOG. This finding indicates that HMGA2 is a pulpal stem cell regulatory factor. In addition to this, we discovered that several proliferation-related genes, including CDC2A and CDK4, were up-regulated in deciduous pulp cells, while matrix
genes COL1A1, fibronectin and several signaling molecules, such as VEGF, FGFr-1 and IGFr-1 were up-regulated in the pulp cells from permanent teeth.
Conclusions: Taken together, our data suggest that deciduous pulp cells are more robust in self- renewal and proliferation, whereas adult dental pulp cells are more capable of signaling and matrix synthesis.

Files

  • thumnail for 13287_2015_Article_56.pdf 13287_2015_Article_56.pdf binary/octet-stream 2.84 MB Download File

Also Published In

Title
Stem Cell Research & Therapy
DOI
https://doi.org/10.1186/s13287-015-0056-7

More About This Work

Academic Units
Medicine
College of Physicians and Surgeons
Publisher
BioMed Central
Published Here
July 31, 2015
Academic Commons provides global access to research and scholarship produced at Columbia University, Barnard College, Teachers College, Union Theological Seminary and Jewish Theological Seminary. Academic Commons is managed by the Columbia University Libraries.