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Next generation Advanced Laser Fluorometry (ALF) for characterization of natural aquatic environments: new instruments

Chekalyuk, Alexander M.; Hafez, Mark A.

The new optical design allows single- or multi-wavelength excitation of laser-stimulated emission (LSE), provides optimized LSE optical collection for spectral and temporal analyses, and incorporates swappable modules for flow-through and small-volume sample measurements. The basic instrument configuration uses 510 nm laser excitation for assessments of chlorophyll-a, phycobiliprotein pigments, variable fluorescence (F_v/F_m) and chromophoric dissolved organic matter (CDOM) in CDOM-rich waters. The three-laser instrument configuration (375, 405, and 510 nm excitation) provides additional Fv/Fm measurements with 405 nm excitation, CDOM assessments in a broad concentration range, and potential for spectral discrimination between oil and CDOM fluorescence. The new measurement protocols, analytical algorithms and examples of laboratory and field measurements are discussed.

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Also Published In

Title
Optics Express
DOI
https://doi.org/10.1364/OE.21.014181

More About This Work

Academic Units
Lamont-Doherty Earth Observatory
Biology and Paleo Environment
Published Here
July 9, 2013