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Molecular Detection of Leptospiral DNA in Environmental Water on St. Kitts

Rawlins, Julienne; Portanova, Alexandra; Zuckerman, Ilana; Loftis, Amanda; Ceccato, Pietro N.; Willingham, Arve Lee; Verma, Ashutosh

Leptospirosis is an important waterborne zoonotic disease caused by pathogenic Leptospira. The pathogen is maintained in a population due to chronic colonization and shedding from renal tubules of domestic and wild animals. Humans and other animals become infected when they come in contact with urine from infected animals, either directly or through urine-contaminated surface water. In this study, we screened environmental water on the island of St. Kitts by using a TaqMan based real time quantitative polymerase chain reaction (qPCR) targeting a pathogen specific leptospiral gene, lipl32. Our results indicate that around one-fifth of tested water sources have detectable leptospiral DNA.

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Title
International Journal of Environmental Research and Public Health
DOI
https://doi.org/10.3390/ijerph110807953

More About This Work

Academic Units
International Research Institute for Climate and Society
Publisher
MDPI
Published Here
February 11, 2016