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RuBi-Glutamate: two-photon and visible-light photoactivation of neurons and dendritic spines

Fino, Elodie; Araya, Roberto; Peterka, Darcy; Salierno, Marcelo; Etchenique, Roberto; Yuste, Rafael

We describe neurobiological applications of RuBi-Glutamate, a novel caged-glutamate compound based on ruthenium photochemistry. RuBi-Glutamate can be excited with visible wavelengths and releases glutamate after one- or two-photon excitation. It has high quantum efficiency and can be used at low concentrations, partly avoiding the blockade of GABAergic transmission present with other caged compounds. Two-photon uncaging of RuBi-glutamate has a high spatial resolution and generates excitatory responses in individual dendritic spines with physiological kinetics. With laser beam multiplexing, RuBi-Glutamate uncaging can also be used to depolarize and fire pyramidal neurons with single-cell resolution. RuBi-Glutamate therefore enables the photo-activation of neuronal dendrites and circuits with visible or two-photon light sources, achieving single spine, or single cell, precision.

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Published In
Frontiers in Neural Circuits
Publisher DOI
https://doi.org/10.3389/neuro.04.002.2009
Volume
3
Issue
2
Academic Units
Biological Sciences
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