2009 Articles
RuBi-Glutamate: two-photon and visible-light photoactivation of neurons and dendritic spines
We describe neurobiological applications of RuBi-Glutamate, a novel caged-glutamate compound based on ruthenium photochemistry. RuBi-Glutamate can be excited with visible wavelengths and releases glutamate after one- or two-photon excitation. It has high quantum efficiency and can be used at low concentrations, partly avoiding the blockade of GABAergic transmission present with other caged compounds. Two-photon uncaging of RuBi-glutamate has a high spatial resolution and generates excitatory responses in individual dendritic spines with physiological kinetics. With laser beam multiplexing, RuBi-Glutamate uncaging can also be used to depolarize and fire pyramidal neurons with single-cell resolution. RuBi-Glutamate therefore enables the photo-activation of neuronal dendrites and circuits with visible or two-photon light sources, achieving single spine, or single cell, precision.
Subjects
Files
- rubiglutamate_paper.pdf application/pdf 3.43 MB Download File
Also Published In
- Title
- Frontiers in Neural Circuits
- DOI
- https://doi.org/10.3389/neuro.04.002.2009
More About This Work
- Academic Units
- Biological Sciences
- Published Here
- September 9, 2011