2016 Articles
A High-Throughput Strategy for Dissecting Mammalian Genetic Interactions
Comprehensive delineation of complex cellular networks requires high-throughput interrogation of genetic interactions. To address this challenge, we describe the development of a multiplex combinatorial strategy to assess pairwise genetic interactions using CRISPR-Cas9 genome editing and next-generation sequencing. We characterize the performance of combinatorial genome editing and analysis using different promoter and gRNA designs and identified regions of the chimeric RNA that are compatible with next-generation sequencing preparation and quantification. This approach is an important step towards elucidating genetic networks relevant to human diseases and the development of more efficient Cas9-based therapeutics.
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Also Published In
- Title
- PLoS ONE
- DOI
- https://doi.org/10.1371/journal.pone.0167617
More About This Work
- Academic Units
- Systems Biology
- Biochemistry and Molecular Biophysics
- Microbiology and Immunology
- Pathology and Cell Biology
- Publisher
- Public Library of Science
- Published Here
- February 8, 2017