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Enzymatic cleavage of histone H3: a new consideration when measuring histone modifications in human samples

Howe, Caitlin Grace; Gamble, Mary

Histone modifications are increasingly being used as biomarkers of cancer prognosis and survival. However, we identified a cleavage product of histone H3 in human peripheral blood mononuclear cells, which interferes with measures of certain H3 modifications. Therefore, the potential for enzymatic cleavage of histones should be considered when measuring histone modifications in human samples. Furthermore, the enzymatic cleavage of human H3 is itself a fascinating area of research and two important questions remain to be answered: 1) Does cleavage of human H3 occur in vivo, as it does in other organisms? and 2) Does it serve a biologically important function?

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Also Published In

Title
Clinical Epigenetics
DOI
https://doi.org/10.1186/s13148-014-0041-5

More About This Work

Academic Units
Environmental Health Sciences
Publisher
BioMed Central
Published Here
January 26, 2015