Academic Commons

Articles

RNA sequencing from human neutrophils reveals distinct transcriptional differences associated with chronic inflammatory states

Jiang, Kaiyu; Sun, Xiaoyun; Chen, Yanmin; Shen, Yufeng; Jarvis, James N.

Background
The transcriptional complexity of mammalian cells suggests that they have broad abilities to respond to specific environmental stimuli and physiologic contexts. These abilities were not apparent a priori from the structure of mammalian genomes, but have been identified through detailed transcriptome analyses. In this study, we examined the transcriptomes of cells of the innate immune system, human neutrophils, using RNA sequencing (RNAseq).

Methods
We sequenced poly-A RNA from nine individual samples corresponding to specific phenotypes: three children with active, untreated juvenile idiopathic arthritis (JIA)(AD), three children with the same disease whose disease was inactive on medication (CRM), and three children with cystic fibrosis (CF).

Results
We demonstrate that transcriptomes of neutrophils, typically considered non-specific in their responses and functions, display considerable specificity in their transcriptional repertoires dependent on the pathologic context, and included genes, gene isoforms, and long non-coding RNA transcripts. Furthermore, despite the small sample numbers, these findings demonstrate the potential of RNAseq approaches to biomarker development in rheumatic diseases.

Conclusions
These data demonstrate the capacity of cells previously considered non-specific in function to adapt their transcriptomes to specific biologic contexts. These data also provide insight into previously unrecognized pathological pathways and show considerable promise for elucidating disease and disease-state specific regulatory networks.

Files

  • thumnail for 12920_2015_Article_128.pdf 12920_2015_Article_128.pdf binary/octet-stream 1.73 MB Download File

Also Published In

Title
BMC Medical Genomics
DOI
https://doi.org/10.1186/s12920-015-0128-7

More About This Work

Academic Units
Systems Biology
Published Here
August 27, 2015
Academic Commons provides global access to research and scholarship produced at Columbia University, Barnard College, Teachers College, Union Theological Seminary and Jewish Theological Seminary. Academic Commons is managed by the Columbia University Libraries.