Academic Commons

Articles

Derivation of Two New Human Embryonic Stem Cell Lines from Nonviable Human Embryos

Vunjak-Novakovic, Gordana; Gavrilov, Svetlana; Marolt, Darja; Douglas, Nataki C.; Prosser, Robert W.; Khalid, Imran; Sauer, Mark V.; Landry, Donald W.; Papaioannou, Virginia E.

We report the derivation and characterization of two new human embryonic stem cells (hESC) lines (CU1 and CU2) from embryos with an irreversible loss of integrated organismic function. In addition, we analyzed retrospective data of morphological progression from embryonic day (ED) 5 to ED6 for 2480 embryos not suitable for clinical use to assess grading criteria indicative of loss of viability on ED5. Our analysis indicated that a large proportion of in vitro fertilization (IVF) embryos not suitable for clinical use could be used for hESC derivation. Based on these combined findings, we propose that criteria commonly used in IVF clinics to determine optimal embryos for uterine transfer can be employed to predict the potential for hESC derivation from poor quality embryos without the destruction of vital human embryos.

Files

Also Published In

Title
Stem Cells International
DOI
https://doi.org/10.4061/2011/765378

More About This Work

Academic Units
Biomedical Engineering
Publisher
Hindawi Publishing Corporation
Published Here
January 31, 2014
Academic Commons provides global access to research and scholarship produced at Columbia University, Barnard College, Teachers College, Union Theological Seminary and Jewish Theological Seminary. Academic Commons is managed by the Columbia University Libraries.