A Microrobotic System For Protein Streak Seeding

Georgiev, Atanas; Allen, Peter K.; Song, Ting; Laine, Andrew F.; Edstrom, William; Hunt, John F.

We present a microrobotic system for protein crystal micromanipulation tasks. The focus in this report is on a task called streak seeding, which is used by crystallographers to entice certain protein crystals to grow. Our system features a set of custom designed micropositioner end-effectors we call microshovels to replace traditional tools used by crystallographers for this task. We have used micro-electrical mechanical system (MEMS) techniques to design and manufacture various shapes and quantities of microshovels. Visual feedback from a camera mounted on the microscope is used to control the micropositioner as it lowers a microshovel into the liquid containing the crystals for poking and streaking. We present experimental results that illustrate the applicability of our approach.



More About This Work

Academic Units
Computer Science
Department of Computer Science, Columbia University
Columbia University Computer Science Technical Reports, CUCS-041-04
Published Here
April 22, 2011