2003 Articles

Conserved pathways within bacteria and yeast as revealed by global protein network alignment

Kelly, Brian P.; Sharan, Roded; Karp, Richard; Sittler, Taylor; Root, David; Stockwell, Brent R.; Ideker, Trey

We implement a strategy for aligning two protein–protein interaction networks that combines interaction topology and protein sequence similarity to identify conserved interaction pathways and complexes. Using this approach we show that the protein–protein interaction networks of two distantly related species, Saccharomyces cerevisiae and Helicobacter pylori, harbor a large complement of evolutionarily conserved pathways, and that a large number of pathways appears to have duplicated and specialized within yeast. Analysis of these findings reveals many well characterized interaction pathways as well as many unanticipated pathways, the significance of which is reinforced by their presence in the networks of both species.