Non-invasive, Focused Ultrasound-Facilitated Gene Delivery for Optogenetics

Wang, Shutao; Kugelman, Tara; Buch, Amanda M.; Herman, Mathieu; Han, Yang; Karakatsani, Maria Eleni; Hussaini, Syed Abid; Duff, Karen E.; Konofagou, Elisa E.

Optogenetics, a widely used technique in neuroscience research, is often limited by its invasive nature of application. Here, we present a noninvasive, ultrasound-based technique to introduce optogenetic channels into the brain by temporarily opening the blood-brain barrier (BBB). We demonstrate the efficiency of the method developed and evaluate the bioactivity of the non-invasively introduced channelrhodopsin channels by performing stimulation in freely behaving mice.


Also Published In

Scientific Reports

More About This Work

Academic Units
Biomedical Engineering
Pathology and Cell Biology
Published Here
August 27, 2017