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Nonmuscle Myosin IIA-Dependent Force Inhibits Cell Spreading and Drives F-Actin Flow

Cai, Yunfei; Biais, Nicolas; Giannone, Gregory; Tanase, Monica; Jiang, Guoying; Hoffman, Jake M.; Wiggins, Chris H.; Silberzan, Pascal; Buguin, Alex; Ladoux, Benoit; Sheetz, Michael P.

Nonmuscle myosin IIA (NMM-IIA) is involved in the formation of focal adhesions and neurite retraction. However, the role of NMM-IIA in these functions remains largely unknown. Using RNA interference as a tool to decrease NMM-IIA expression, we have found that NMM-IIA is the major myosin involved in traction force generation and retrograde F-actin flow in mouse embryonic fibroblast cells. Quantitative analyses revealed that ∼60% of traction force on fibronectin-coated surfaces is contributed by NMM-IIA and ∼30% by NMM-IIB. The retrograde F-actin flow decreased dramatically in NMM-IIA-depleted cells, but seemed unaffected by NMM-IIB deletion. In addition, we found that depletion of NMM-IIA caused cells to spread at a higher rate and to a greater area on fibronectin substrates during the early spreading period, whereas deletion of NMM-IIB appeared to have no effect on spreading. The distribution of NMM-IIA was concentrated on the dorsal surface and approached the ventral surface in the periphery, whereas NMM-IIB was primarily concentrated around the nucleus and to a lesser extent at the ventral surface in cell periphery. Our results suggest that NMM-IIA is involved in generating a coherent cytoplasmic contractile force from one side of the cell to the other through the cross-linking and the contraction of dorsal actin filaments.

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Also Published In

Title
Biophysical Journal
DOI
https://doi.org/10.1529/biophysj.106.084806

More About This Work

Academic Units
Biological Sciences
Publisher
Cell Press
Published Here
September 19, 2014