1974 Articles

Two Classes Of Revertanțs Isolated frpm SV40-transformed 3T3 Mouse Cells

Vogel, Arthur; Oey, Jan; Pollack, Robert

Growth Properties of Serum Revertants Normal and transformed cells show markedly different responses to 1% calf serum (Table 1). Normal 3T3 cells grow poorly in 1% calf serum with a doubling time of 90 hours, whereas SV101 grows relatively well with a doubling time of 30 hours. Serum revertants are isolated by plating SV101 in either 1% calf serum or 10% agamma-depleted calf serum (Smith et al. 1971) and adding BrdU to kill dividing cells (Vogel and Pollack 1973). Revertants isolated in 1% calf serum are designated as LsSV, and revertants isolated in 10% agamma-depleted serum are designated as AySV. Neither type of serum revertant can grow at all in 1% calf serum (Fig. I).