Changes in the biochemical constituents and morphologic appearance of the human cervical stroma during pregnancy

Kristin M. Myers; Simona Socrate; Dimitrios Tzeranis; Michael House

Changes in the biochemical constituents and morphologic appearance of the human cervical stroma during pregnancy
Myers, Kristin M.
Socrate, Simona
Tzeranis, Dimitrios
House, Michael
Mechanical Engineering
Permanent URL:
Book/Journal Title:
European Journal of Obstetrics & Gynecology and Reproductive Biology
Objective: The cervix is the lower portion of the uterus. It is composed of fibrous tissue and its mechanical integrity is crucial for maintaining a healthy gestation. During normal pregnancy, the cervical extracellular matrix (ECM) remodels in preparation for labor. The objective of this study was to investigate the biochemical and morphological changes in cervical stroma associated with physiological remodeling during normal pregnancy. Study design: Using human cervical tissue obtained from pregnant and non-pregnant patients, the ECM was analyzed for its biochemical constituents and histologic morphology. The ECM was assayed for hydration, collagen concentration, collagen solubility, total sulfated glycosaminoglycan concentration, and individual disaccharide concentration. The ECM morphology was visualized using conventional histological techniques (Masson's trichrome stain, polarized light microscopy) as well as second harmonic generation (SHG) imaging. Results: When comparing pregnant to non-pregnant tissue, significant increases were measured for total sulfated glycosaminoglycans, hyaluronic acid, and collagen solubility. The microscopy studies confirmed that the collagenous network of the cervical stroma was anisotropic and pregnancy was associated with a discernable decrease in collagen organization. Conclusion: Significant changes were seen in the concentration and organization of cervical ECM constituents during normal pregnancy.
Obstetrics and gynecology
Publisher DOI:
Item views:
text | xml

In Partnership with the Center for Digital Research and Scholarship at Columbia University Libraries/Information Services | Terms of Use