Dissertations:
Functional Imaging Through Dark State Dynamics
Evangelos George Gatzogiannis
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- Title:
- Functional Imaging Through Dark State Dynamics
- Author(s):
- Gatzogiannis, Evangelos George
- Thesis Advisor(s):
- Min, Wei
- Date:
- 2011
- Type:
- Dissertations
- Department:
- Chemistry
- Permanent URL:
- http://hdl.handle.net/10022/AC:P:11855
- Notes:
- Ph.D., Columbia University.
- Abstract:
- This thesis harnesses the environmental sensitivity of the dark states of molecular fluorophores, both endogeneous in cells/tissue and externally introduced for mapping of chemical micro-environments including factors such as ion concentration and microviscosity. A novel technique for directly detecting the dynamics and population of dark states, such as the lowest triplet state, was developed and called FAPA, Fluorescence Anomalous Phase Advance. This technique is a sensitive and fast reporter of dark state dynamics and can be used for imaging. In addition, a genetically-encoded microviscosity and micro-environmental mapping ability with high protein-specific specificity was developed by using a TMP-tag and Fluorescence Lifetime Imaging.
- Subject(s):
- Physical chemistry
- Item views:
- 91