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Functional Imaging Through Dark State Dynamics

Evangelos George Gatzogiannis

Title:
Functional Imaging Through Dark State Dynamics
Author(s):
Gatzogiannis, Evangelos George
Thesis Advisor(s):
Min, Wei
Date:
Type:
Dissertations
Department:
Chemistry
Permanent URL:
Notes:
Ph.D., Columbia University.
Abstract:
This thesis harnesses the environmental sensitivity of the dark states of molecular fluorophores, both endogeneous in cells/tissue and externally introduced for mapping of chemical micro-environments including factors such as ion concentration and microviscosity. A novel technique for directly detecting the dynamics and population of dark states, such as the lowest triplet state, was developed and called FAPA, Fluorescence Anomalous Phase Advance. This technique is a sensitive and fast reporter of dark state dynamics and can be used for imaging. In addition, a genetically-encoded microviscosity and micro-environmental mapping ability with high protein-specific specificity was developed by using a TMP-tag and Fluorescence Lifetime Imaging.
Subject(s):
Physical chemistry
Item views:
141
Metadata:
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