Induction of sterilizing immunity against West Nile Virus (WNV), by immunization with WNV-like particles produced in insect cells

Articles:

Title:: Induction of sterilizing immunity against West Nile Virus (WNV), by immunization with WNV-like particles produced in insect cells
Author(s):: Qiao, Ming
Ashok, Mundrigi
Bernard, Kristen A.
Palacios, Gustavo F.
Zhou, Z. Hong
Lipkin, W. Ian
Liang, T. Jake
Date:: 2004
Type:: Articles
Department:: Center for Infection and Immunity
Volume:: 190
Permanent URL:: http://hdl.handle.net/10022/AC:P:9996
Book/Journal Title:: Journal of infectious diseases
Abstract:: No specific vaccine for West Nile virus (WNV) is currently available for human use. In the present study, we describe the generation of WNV-like particles (WNV-LPs) in insect cells by use of recombinant baculoviruses expressing the WNV structural proteins prME or CprME. BALB/c mice immunized with purified WNV-LPs developed WNV-specific antibodies that had potent neutralizing activities. Mice immunized with prME-like particles (prME-LPs) showed no morbidity or mortality after challenge with WNV. Immunization with prMELPs can induce sterilizing immunity without producing any evidence of viremia or viral RNA in the spleen or brain. These results suggest that WNV-LPs hold promise as a vaccine candidate for WNV infection.
Subject(s):: Epidemiology
Virology
DOI:: 10.1086/425933