Home

Genetic and epidemiological characterization of Stretch Lagoon orbivirus, a novel orbivirus isolated from Culex and Aedes mosquitoes in northern Australia

Chris Cowled; Gustavo F. Palacios; Lorna Melville; Richard Weir; Susan Walsh; Steven Davis; Aneta Gubala; W. Ian Lipkin; Thomas Briese; David Boyle

Title:
Genetic and epidemiological characterization of Stretch Lagoon orbivirus, a novel orbivirus isolated from Culex and Aedes mosquitoes in northern Australia
Author(s):
Cowled, Chris
Palacios, Gustavo F.
Melville, Lorna
Weir, Richard
Walsh, Susan
Davis, Steven
Gubala, Aneta
Lipkin, W. Ian
Briese, Thomas
Boyle, David
Date:
Type:
Articles
Department:
Center for Infection and Immunity
Volume:
90
Permanent URL:
Book/Journal Title:
Journal of general virology
Abstract:
Stretch Lagoon orbivirus (SLOV) was isolated in 2002 from pooled Culex annulirostris mosquitoes collected at Stretch Lagoon, near the Wolfe Creek national park in the Kimberley region of Western Australia. Conventional serological tests were unable to identify the isolate, and electron microscopy indicated a virus of the genus Orbivirus, family Reoviridae. Here, a cDNA subtraction method was used to obtain approximately one-third of the viral genome, and further sequencing was performed to complete the sequences of segment 1 (viral polymerase) and segment 2 (conserved inner-core protein). Phylogenetic analysis showed that SLOV should be considered a new species within the genus Orbivirus. A real-time RT-PCR test was designed to study the epidemiology of SLOV in the field. Six additional isolates of SLOV were identified, including isolates from four additional locations and two additional mosquito species. Horses, donkeys and goats were implicated as potential vertebrate hosts in a serological survey.
Subject(s):
Epidemiology
Virology
Publisher DOI:
http://dx.doi.org/10.1099/vir.0.010074-0
Item views:
206
Metadata:
text | xml

In Partnership with the Center for Digital Research and Scholarship at Columbia University Libraries/Information Services | Terms of Use