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Characterization of the major nuclear localization signal of the Borna disease virus phosphoprotein

Martin Schwemmle; Christian Jehle; Trevor Shoemaker; W. Ian Lipkin

Title:
Characterization of the major nuclear localization signal of the Borna disease virus phosphoprotein
Author(s):
Schwemmle, Martin
Jehle, Christian
Shoemaker, Trevor
Lipkin, W. Ian
Date:
Type:
Articles
Department:
Center for Infection and Immunity
Volume:
80
Permanent URL:
Book/Journal Title:
Journal of general virology
Abstract:
Borna disease virus (BDV) replicates and transcribes its negative-sense RNA genome in the nucleus. The BDV phosphoprotein (P) is localized in the nucleus of infected cells and cells transfected with P expression constructs. To identify the nuclear localization signal (NLS) of P, COS- 7 cells were transfected with wild-type or mutant forms of P fused with green fluorescent protein (GFP). Whereas GFP alone was exclusively cytoplasmic, P or P-GFP were nuclear. Analysis of carboxy- and amino- terminal truncation mutants of P indicated that amino acids (aa) 20-37 are sufficient to promote efficient nuclear accumulation of the fusion protein. Residual nuclear import of GFP was observed with portions of P including aa 33-134 or aa 134-201, suggesting the presence of additional NLS motifs. The major NLS of P appears to be bipartite. It consists of two basic aa domains, R22RER25 and R30PRKIPR36, separated by four non-basic aa, S26GSP29.
Subject(s):
Epidemiology
Virology
Item views:
370
Metadata:
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