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Newly discovered Ebola virus associated with hemorrhagic fever outbreak in Uganda

Jonathan S. Towner; Tara K. Sealy; Marina L. Khristova; César G. Albariñ; Sean Conlan; Serena A. Reeder; Phuong-Lan Quan; W. Ian Lipkin; Robert Downing; Jordan W. Tappero; Samuel Okware; Julius Lutwama; Barnabas Bakamutumaho; John Kayiwa; James A. Comer; Pierre E. Rollin; Thomas G. Ksiazek; Stuart T. Nichol

Title:
Newly discovered Ebola virus associated with hemorrhagic fever outbreak in Uganda
Author(s):
Towner, Jonathan S.
Sealy, Tara K.
Khristova, Marina L.
Albariñ, César G.
Conlan, Sean
Reeder, Serena A.
Quan, Phuong-Lan
Lipkin, W. Ian
Downing, Robert
Tappero, Jordan W.
Okware, Samuel
Lutwama, Julius
Bakamutumaho, Barnabas
Kayiwa, John
Comer, James A.
Rollin, Pierre E.
Ksiazek, Thomas G.
Nichol, Stuart T.
Date:
Type:
Articles
Department:
Center for Infection and Immunity
Volume:
4
Permanent URL:
Book/Journal Title:
PLoS pathogens
Abstract:
In this report we describe a newly discovered ebolavirus species which caused a large hemorrhagic fever outbreak in western Uganda. The virus is genetically distinct, differing by more than 30% at the genome level from all other known ebolavirus species. The unique nature of this virus created challenges for traditional filovirus molecular based diagnostic assays and genome sequencing approaches. Instead, we quickly determined over 70% of the virus genome using a recently developed random-primed pyrosequencing approach that allowed the rapid development of a molecular detection assay that was deployed in the disease outbreak response. This draft sequence allowed easy completion of the whole genome sequence using a traditional primer walking approach and prompt confirmation that this virus represented a new ebolavirus species. Current efforts to design effective diagnostics, antivirals and vaccines will need to take into account the distinct nature of this important new member of the filovirus family.
Subject(s):
Epidemiology
Virology
Publisher DOI:
10.1371/journal.ppat.1000212
Item views:
413
Metadata:
text | xml

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