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Panmicrobial oligonucleotide array for diagnosis of infectious diseases

Gustavo F. Palacios; Phuong-Lan Quan; Omar J. Jabado; Sean Conlan; David L. Hirschberg; Yang Liu; Junhui Zhai; Neil Renwick; Jeffrey Hui; Hedi Hegyi; Allen Grolla; James E. Strong; Jonathan S. Towner; Thomas W. Geisbert; Peter B. Jahrling; Cornelia Büchen-Osmond; Heinz Ellerbrok; Maria Paz Sanchez-Seco; Yves Lussier; Pierre Formenty; Stuart T. Nichol; Heinz Feldmann; Thomas Briese; W. Ian Lipkin

Title:
Panmicrobial oligonucleotide array for diagnosis of infectious diseases
Author(s):
Palacios, Gustavo F.
Quan, Phuong-Lan
Jabado, Omar J.
Conlan, Sean
Hirschberg, David L.
Liu, Yang
Zhai, Junhui
Renwick, Neil
Hui, Jeffrey
Hegyi, Hedi
Grolla, Allen
Strong, James E.
Towner, Jonathan S.
Geisbert, Thomas W.
Jahrling, Peter B.
Büchen-Osmond, Cornelia
Ellerbrok, Heinz
Sanchez-Seco, Maria Paz
Lussier, Yves
Formenty, Pierre
Nichol, Stuart T.
Feldmann, Heinz
Briese, Thomas
Lipkin, W. Ian
Date:
Type:
Articles
Department:
Center for Infection and Immunity
Volume:
13
Permanent URL:
Book/Journal Title:
Emerging infectious diseases
Abstract:
To facilitate rapid, unbiased, differential diagnosis of infectious diseases, we designed GreeneChipPm, a panmicrobial microarray comprising 29,455 sixty-mer oligonucleotide probes for vertebrate viruses, bacteria, fungi, and parasites. Methods for nucleic acid preparation, random primed PCR amplification, and labeling were optimized to allow the sensitivity required for application with nucleic acid extracted from clinical materials and cultured isolates. Analysis of nasopharyngeal aspirates, blood, urine, and tissue from persons with various infectious diseases confirmed the presence of viruses and bacteria identified by other methods, and implicated Plasmodium falciparum in an unexplained fatal case of hemorrhagic feverlike disease during the Marburg hemorrhagic fever outbreak in Angola in 2004–2005.
Subject(s):
Epidemiology
Virology
Item views:
280
Metadata:
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