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Use of avian bornavirus isolates to induce proventricular dilatation disease in conures

Patricia Gray; Sharman Hoppes; Paulette Suchodolski; Negin Mirhosseini; Susan Payne; Itamar Villanueva; H. L. Shivaprasad; Kirsi S. Honkavuori; Thomas Briese; Sanjay M. Reddy; Ian Tizard

Title:
Use of avian bornavirus isolates to induce proventricular dilatation disease in conures
Author(s):
Gray, Patricia
Hoppes, Sharman
Suchodolski, Paulette
Mirhosseini, Negin
Payne, Susan
Villanueva, Itamar
Shivaprasad, H. L.
Honkavuori, Kirsi S.
Briese, Thomas
Reddy, Sanjay M.
Tizard, Ian
Date:
Type:
Articles
Department:
Center for Infection and Immunity
Volume:
16
Permanent URL:
Book/Journal Title:
Emerging infectious diseases
Abstract:
Avian bornavirus (ABV) is a newly discovered member of the family Bornaviridae that has been associated with the development of a lethal neurologic syndrome in birds, termed proventricular dilatation disease (PDD). We successfully isolated and characterized ABV from the brains of 8 birds with confirmed PDD. One isolate was passed 6 times in duck embryo fibroblasts, and the infected cells were then injected intramuscularly into 2 healthy Patagonian conures (Cyanoliseus patagonis). Clinical PDD developed in both birds by 66 days postinfection. PDD was confirmed by necropsy and histopathologic examination. Reverse transcription–PCR showed that the inoculated ABV was in the brains of the 2 infected birds. A control bird that received uninfected tissue culture cells remained healthy until it was euthanized at 77 days. Necropsy and histopathologic examinations showed no abnormalities; PCR did not indicate ABV in its brain tissues.
Subject(s):
Epidemiology
Virology
Publisher DOI:
10.3201/eid1603.091257
Item views:
190
Metadata:
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