Home

RuBi-Glutamate: two-photon and visible-light photoactivation of neurons and dendritic spines

Elodie Fino; Roberto Araya; Darcy Peterka; Marcelo Salierno; Roberto Etchenique; Rafael Yuste

Title:
RuBi-Glutamate: two-photon and visible-light photoactivation of neurons and dendritic spines
Author(s):
Fino, Elodie
Araya, Roberto
Peterka, Darcy
Salierno, Marcelo
Etchenique, Roberto
Yuste, Rafael
Date:
Type:
Articles
Department:
Biological Sciences
Volume:
3
Permanent URL:
Book/Journal Title:
Frontiers in Neural Circuits
Abstract:
We describe neurobiological applications of RuBi-Glutamate, a novel caged-glutamate compound based on ruthenium photochemistry. RuBi-Glutamate can be excited with visible wavelengths and releases glutamate after one- or two-photon excitation. It has high quantum efficiency and can be used at low concentrations, partly avoiding the blockade of GABAergic transmission present with other caged compounds. Two-photon uncaging of RuBi-glutamate has a high spatial resolution and generates excitatory responses in individual dendritic spines with physiological kinetics. With laser beam multiplexing, RuBi-Glutamate uncaging can also be used to depolarize and fire pyramidal neurons with single-cell resolution. RuBi-Glutamate therefore enables the photo-activation of neuronal dendrites and circuits with visible or two-photon light sources, achieving single spine, or single cell, precision.
Subject(s):
Neurosciences
Publisher DOI:
10.3389/neuro.04.002.2009
Item views:
329
Metadata:
text | xml

In Partnership with the Center for Digital Research and Scholarship at Columbia University Libraries/Information Services | Terms of Use