Analysis of Spectral Excitation for Measurements of Fluorescence Constituents in Natural Waters

Chekalyuk, Alexander M.; Hafez, Mark A.

Field measurements of chlorophyll-a (Chl), phycoerythrin (PE), chromophoric dissolved organic matter (CDOM), and variable fluorescence (Fv/Fm) in diverse waters of the California Current, Mediterranean Sea and Gulf of Mexico using 375, 405, 510 and 532 nm laser excitation wavelengths (EW) are analyzed. EW = 375 and 405 nm were found more suitable for Chl assessment in high-Chl (greater than 10 μg/l) waters. Both EW = 532 and 510 nm can be used to efficiently stimulate PE fluorescence for structural characterization of phytoplankton communities. EW = 375 nm and 405 nm can provide best results for CDOM assessments in offshore oceanic waters; the green EWs can be also used for CDOM measurements in fresh and estuarine water types in conjunction with spectral discrimination between CDOM and PE fluorescence. Both EW = 405 and 510 are suitable for photo-physiological Fv/Fm assessments, though using EW = 405 nm may result in underestimation of PE-containing phytoplankton groups present in mixed phytoplankton assemblages.



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Also Published In

Optics Express

More About This Work

Academic Units
Lamont-Doherty Earth Observatory
Biology and Paleo Environment
The Optical Society
Published Here
January 24, 2014


This paper was published in Optics Express and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law.