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Macroautophagy Abnormality in Essential Tremor

Sheng-Han Kuo; Guomei Tang; Karen Ma; Rachel Babij; Etty Cortes; Jean Paul Vonsattel; Phyllis L. Faust; David L. Sulzer; Elan D. Louis

Title:
Macroautophagy Abnormality in Essential Tremor
Author(s):
Kuo, Sheng-Han
Tang, Guomei
Ma, Karen
Babij, Rachel
Cortes, Etty
Vonsattel, Jean Paul
Faust, Phyllis L.
Sulzer, David L.
Louis, Elan D.
Date:
Type:
Articles
Department(s):
Neurology
Pathology and Cell Biology
Taub Institute
Sergievsky Center
Volume:
7
Persistent URL:
Book/Journal Title:
PLOS ONE
Abstract:
Macroautophagy is a cellular mechanism for the clearance of protein aggregates and damaged organelles. Impaired macroautophagy has been observed in neurodegenerative disorders. We investigated the macroautophagy pathway in essential tremor (ET) cases compared to age-matched controls. We analyzed microtubule-associated protein light chain 3-II (LC3-II), S6K, phosphorylated S6K, beclin-1, and mitochondrial membrane proteins levels by Western blot in the post-mortem cerebellum of 10 ET cases and 11 controls. We also performed immunohistochemistry in 12 ET cases and 13 controls to quantify LC3 clustering in Purkinje cells (PCs). LC3-II protein levels were significantly lower in ET cases vs. controls on Western blot (0.84±0.14 vs. 1.00±0.14, p = 0.02), and LC3-II clustering in PCs by immunohistochemistry was significantly lower in ET cases vs. controls (2.03±3.45 vs. 8.80±9.81, p = 0.03). In ET cases, disease duration was inversely correlated with LC3-II protein level (r = −0.64, p = 0.046). We found that mitochondrial membrane proteins were accumulated in ET (TIM23: 1.36±0.11 in ET cases vs. 1.00±0.08 in controls, p = 0.02; TOMM20: 1.63±0.87 in ET cases vs. 1.00±0.14 in controls, p = 0.03). Beclin-1, which is involved in macroautophagy, was strikingly deficient in ET (0.42±0.13 vs. 1.00±0.35, p<0.001). Decreased macroautophagy was observed in the ET cerebellum, and this could be due to a decrease in beclin-1 levels, which subsequently lead to mitochondrial accumulation as a result of autophagic failure. This provides a possible means by which perturbed macroautophagy could contribute to PC pathology in ET.
Subject(s):
Neurology
Tremor
Movement disorders
Immunohistochemistry--Technique
Publisher DOI:
https://doi.org/10.1371/journal.pone.0053040
Item views
20
Metadata:
text | xml
Suggested Citation:
Sheng-Han Kuo, Guomei Tang, Karen Ma, Rachel Babij, Etty Cortes, Jean Paul Vonsattel, Phyllis L. Faust, David L. Sulzer, Elan D. Louis, , Macroautophagy Abnormality in Essential Tremor, Columbia University Academic Commons, .

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