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Inhibition of NF-κB improves the stress resistance and myogenic differentiation of MDSPCs isolated from naturally aged mice

Jonathan D. Proto; Aiping Lu; Akaitz Dorronsoro; Alex Scibetta; Paul Robbins; Laura J. Niedernhofer; Johnny Huard

Title:
Inhibition of NF-κB improves the stress resistance and myogenic differentiation of MDSPCs isolated from naturally aged mice
Author(s):
Proto, Jonathan D.
Lu, Aiping
Dorronsoro, Akaitz
Scibetta, Alex
Robbins, Paul
Niedernhofer, Laura J.
Huard, Johnny
Date:
Type:
Articles
Department(s):
Medicine
Volume:
12
Persistent URL:
Book/Journal Title:
PLoS ONE
Abstract:
A decline in the regenerative capacity of adult stem cells with aging is well documented. As a result of this decline, the efficacy of autologous stem cell therapies is likely to decline with increasing donor age. In these cases, strategies to restore the function of aged stem cells would have clinical utility. Globally, the transcription factor NF-κB is up-regulated in aged tissues. Given the negative role that NF-κB plays in myogenesis, we investigated whether the age-related decline in the function of muscle-derived stem/progenitor cells (MDSPCs) could be improved by inhibition of NF-κB. Herein, we demonstrate that pharmacologic or genetic inhibition of NF-κB activation increases myogenic differentiation and improves resistance to oxidative stress. Our results suggest that MDSPC “aging” may be reversible, and that pharmacologic targeting of pathways such as NF-κB may enhance the efficacy of cell-based therapies.
Subject(s):
Stem cells
Transcription factors
Medicine
Aging
Biology
Publisher DOI:
https://doi.org/10.1371/journal.pone.0179270
Item views
45
Metadata:
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Suggested Citation:
Jonathan D. Proto, Aiping Lu, Akaitz Dorronsoro, Alex Scibetta, Paul Robbins, Laura J. Niedernhofer, Johnny Huard, , Inhibition of NF-κB improves the stress resistance and myogenic differentiation of MDSPCs isolated from naturally aged mice, Columbia University Academic Commons, .

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