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A modular yeast biosensor for low-cost point-of-care pathogen detection

Nili Ostrov; Miguel Jimenez; Sonja Billerbeck; James Ronald Brisbois; Joseph Antonio Matragrano; Alastair Kenneth Ager; Virginia W. Cornish

Title:
A modular yeast biosensor for low-cost point-of-care pathogen detection
Author(s):
Ostrov, Nili
Jimenez, Miguel
Billerbeck, Sonja
Brisbois, James Ronald
Matragrano, Joseph Antonio
Ager, Alastair Kenneth
Cornish, Virginia W.
Date:
Type:
Articles
Department(s):
Chemistry
Population and Family Health
Systems Biology
Volume:
3
Persistent URL:
Book/Journal Title:
Science Advances
Abstract:
The availability of simple, specific, and inexpensive on-site detection methods is of key importance for deployment of pathogen surveillance networks. We developed a nontechnical and highly specific colorimetric assay for detection of pathogen-derived peptides based on Saccharomyces cerevisiae—a genetically tractable model organism and household product. Integrating G protein–coupled receptors with a visible, reagent-free lycopene readout, we demonstrate differential detection of major human, plant, and food fungal pathogens with nanomolar sensitivity. We further optimized a one-step rapid dipstick prototype that can be used in complex samples, including blood, urine, and soil. This modular biosensor can be economically produced at large scale, is not reliant on cold-chain storage, can be detected without additional equipment, and is thus a compelling platform scalable to global surveillance of pathogens.
Subject(s):
Biosensors
Chemistry
Yeast
Pathogenic microorganisms--Detection
Synthetic biology
Publisher DOI:
https://doi.org/10.1126/sciadv.1603221
Item views
12
Metadata:
text | xml
Suggested Citation:
Nili Ostrov, Miguel Jimenez, Sonja Billerbeck, James Ronald Brisbois, Joseph Antonio Matragrano, Alastair Kenneth Ager, Virginia W. Cornish, , A modular yeast biosensor for low-cost point-of-care pathogen detection, Columbia University Academic Commons, .

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