HomeHome

An orphan cbb3-type cytochrome oxidase subunit supports Pseudomonas aeruginosa biofilm growth and virulence

Jeanyoung Jo; Krista Lynn Cortez; William-Cole Cornell; Alexa M. Price-Whelan; Lars Dietrich

Title:
An orphan cbb3-type cytochrome oxidase subunit supports Pseudomonas aeruginosa biofilm growth and virulence
Author(s):
Jo, Jeanyoung
Cortez, Krista Lynn
Cornell, William-Cole
Price-Whelan, Alexa M.
Dietrich, Lars
Date:
Type:
Articles
Department(s):
Biological Sciences
Volume:
6
Persistent URL:
Book/Journal Title:
eLife
Abstract:
Hypoxia is a common challenge faced by bacteria during associations with hosts due in part to the formation of densely packed communities (biofilms). cbb3-type cytochrome c oxidases, which catalyze the terminal step in respiration and have a high affinity for oxygen, have been linked to bacterial pathogenesis. The pseudomonads are unusual in that they often contain multiple full and partial (i.e. ‘orphan’) operons for cbb3-type oxidases and oxidase subunits. Here, we describe a unique role for the orphan catalytic subunit CcoN4 in colony biofilm development and respiration in the opportunistic pathogen Pseudomonas aeruginosa PA14. We also show that CcoN4 contributes to the reduction of phenazines, antibiotics that support redox balancing for cells in biofilms, and to virulence in a Caenorhabditis elegans model of infection. These results highlight the relevance of the colony biofilm model to pathogenicity and underscore the potential of cbb3-type oxidases as therapeutic targets.
Subject(s):
Biofilms
Pathogenic bacteria
Pseudomonas aeruginosa
Cytochrome oxidase
Biology
Publisher DOI:
https://doi.org/10.7554/eLife.30205
Item views
23
Metadata:
text | xml
Suggested Citation:
Jeanyoung Jo, Krista Lynn Cortez, William-Cole Cornell, Alexa M. Price-Whelan, Lars Dietrich, , An orphan cbb3-type cytochrome oxidase subunit supports Pseudomonas aeruginosa biofilm growth and virulence, Columbia University Academic Commons, .

Columbia University Libraries | Policies | FAQ