Analysis of the medium (M) segment sequence of Guaroa virus and its comparison to other orthobunyaviruses
- Analysis of the medium (M) segment sequence of Guaroa virus and its comparison to other orthobunyaviruses
- Briese, Thomas
Lipkin, W. Ian
- Center for Infection and Immunity
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- Journal of General Virology
- Guaroa virus (GROV), a segmented virus in the genus Orthobunyavirus, has been linked to the Bunyamwera serogroup (BUN) through cross-reactivity in complement fixation assays of S segment-encoded nucleocapsid protein determinants, and also to the California serogroup (CAL) through cross-reactivity in neutralization assays of M segment-encoded glycoprotein determinants. Phylogenetic analysis of the S-segment sequence supported a closer relationship to the BUN serogroup for this segment and it was hypothesized that the serological reaction may indicate genome-segment reassortment. Here, cloning and sequencing of the GROV M segment are reported. Sequence analysis indicates an organization similar to that of other orthobunyaviruses, with genes in the order GNâ€“nsmâ€“gC, and mature proteins generated by protease cleavage at one, and by signalase at possibly three, sites. A potential role of motifs that are more similar to CAL than to BUN virus sequences with respect to the serological reaction is discussed. No discernable evidence for reassortment was identified.
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- Thomas Briese, Andrew Rambaut, W. Ian Lipkin, 2004, Analysis of the medium (M) segment sequence of Guaroa virus and its comparison to other orthobunyaviruses, Columbia University Academic Commons, https://doi.org/10.7916/D8PN9BDQ.