Fleming Christine P. author Columbia University. Electrical Engineering Ripplinger Crystal M. author Webb Bryan author Efimov Igor R. author Rollins Andrew M. author Columbia University. Electrical Engineering originator text Articles 2008 English Heterogeneity in cardiac tissue microstructure is a potential mechanism for the generation and maintenance of arrhythmias. Abnormal changes in fiber orientation increase the likelihood of arrhythmia. We present optical coherence tomography (OCT) as a method to image myofibers in excised intact heart preparations. Three-dimensional (3-D) image sets were gathered from the rabbit right ventricular free wall (RVFW) using a microscope-integrated OCT system. An automated algorithm for fiber orientation quantification in the plane parallel to the wall surface was developed. The algorithm was validated by comparison with manual measurements. Quantifying fiber orientation in the plane parallel to the wall surface from OCT images can be used to help understand the conduction system of the specific sample being imaged. Medical imaging and radiology 13 3 030505-1 030505-3 2008-05 http://dx.doi.org/10.1117/1.2937470 http://hdl.handle.net/10022/AC:P:15236 NNC NNC 2012-11-08 14:50:17 -0500 2012-11-08 14:57:35 -0500 9238 eng