Visually-guided protein crystal manipulation using micromachined silicon tools
Georgiev
Atanas
author
Columbia University. Computer Science
Edstrom
William
author
Columbia University. Biological Sciences
Allen
Peter K.
author
Columbia University. Computer Science
Columbia University. Computer Science
originator
text
Articles
2004
English
We present a system for protein crystal micro-manipulation with focus on automated crystal mounting for the purposes of X-ray data collection. The system features a set of newly designed micropositioner end-effectors we call microshovels which address some limitations of the traditional cryogenic loops. We have used micro-electrical mechanical system (MEMS) techniques to design and manufacture various shapes and quantities of microshovels. Visual feedback from a camera mounted on the microscope is used to control the micropositioner as it lowers a microshovel into the liquid containing the crystals and approaches a selected crystal for pickup. We present experimental results that illustrate the applicability of our approach.
Nanotechnology
2004 IEEE/RSJ International Conference on Intelligent Robots and Systems (IROS), 28 September - 2 October, 2004, Sendai, Japan: proceedings
Piscataway, N.J.
IEEE
2004
236
241
http://dx.doi.org/10.1109/IROS.2004.1389358
http://hdl.handle.net/10022/AC:P:15184
NNC
NNC
2012-11-05 10:42:03 -0500
2013-04-02 10:50:28 -0400
9186
eng