Schwemmle Martin author Jehle Christian author Shoemaker Trevor author Lipkin W. Ian author Columbia University. Center for Infection and Immunity originator text Articles 1999 English Borna disease virus (BDV) replicates and transcribes its negative-sense RNA genome in the nucleus. The BDV phosphoprotein (P) is localized in the nucleus of infected cells and cells transfected with P expression constructs. To identify the nuclear localization signal (NLS) of P, COS- 7 cells were transfected with wild-type or mutant forms of P fused with green fluorescent protein (GFP). Whereas GFP alone was exclusively cytoplasmic, P or P-GFP were nuclear. Analysis of carboxy- and amino- terminal truncation mutants of P indicated that amino acids (aa) 20-37 are sufficient to promote efficient nuclear accumulation of the fusion protein. Residual nuclear import of GFP was observed with portions of P including aa 33-134 or aa 134-201, suggesting the presence of additional NLS motifs. The major NLS of P appears to be bipartite. It consists of two basic aa domains, R22RER25 and R30PRKIPR36, separated by four non-basic aa, S26GSP29. Epidemiology Virology 80 1 97 100 1999 0022-1317 http://hdl.handle.net/10022/AC:P:9988 NNC Copyright © 1999 by the Society for General Microbiology NNC 2011-03-17 20:04:22 UTC 2011-03-17 20:20:12 UTC 3114 eng